Extraction and Profiling of Phytochemicals Present in Selected Ornamental Plants in Kericho County
Abstract
Bacterial infections are prevalent in most parts of Kenya causing diseases like pneumonia, typhoid, cholera and meningitis. This has contributed to unsustainable socio-economic development following the emergence of antimicrobial resistant strains of bacteria and hence the need for alternative strategies that are not only effective against bacteria but environmentally safe as well. This study was designed to extract and profile phytochemicals present within crude leaves, flowers and roots extracts of Iris versicolor and Nerium oleander for future antimicrobial activities. The plant parts were macerated and extracted to obtain phytochemicals that were then identified under different classes of compounds by treating them with different reagents following standard laboratory procedures. About 500gms of the grounded powder of the parts were soaked in 1.5litres of Hexane, DCM and Methanol and left to settle for 24 hours after which the solutions were filtered with Whatman No. 1 filter paper. The filtrate was then evaporated to a paste using a rotary evaporator equipment. The paste was aliquoted into 20gms and refrigerated for future qualitative analysis. About 2mls of different test solutions were added to 1ml of the plant extracts solutions to obtain tannins, saponins, terpenoids, flavonoids, steroids, phenols and alkaloids. It was found that the test results of the three solvents used for the extraction of the phytochemicals showed gradient elution of the phytochemicals increasing from Hexane, DCM and Methanol. Hexane extracted tannins, flavonoids and phenols while DCM extracted tannins, saponins, flavonoids and alkaloids. Methanol with highest polarity extracted tannins, saponins, terpenoids, flavonoids and phenols. It was also observed that the polarity of the solvent determined the type of bioactive compounds with the most polar extracting different classes of phytochemicals than the less polar solvent. In all considerations, methanolic extract of Nerium oleander leaves and flowers showed positive results for all the phytochemicals present in selected plant extracts
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References
Alorkpa, E. J., Boadi, N. O., Badu, M., & Saah, S. A. (2016). Phytochemical screening, antimicrobial and antioxidant properties of assorted Carica papaya leaves in Ghana.
Augustin, J. M., Kuzina, V., Andersen, S. B., & Bak, S. (2011). Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry, 72(6), 435–457.
Allahverdiyev, A. M., Kon, K. V., Abamor, E. S., Bagirova, M., & Rafailovich, M. (2011). Coping with antibiotic resistance: combining nanoparticles with antibiotics and other antimicrobial agents. Expert Review of Anti-Infective Therapy, 9(11), 1035–1052.
Doughari, J. H. (2012). Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic agents. In Phytochemicals-A global perspective of their role in nutrition and health. InTechOpen.
Duan, L., Zhang, W.-H., Zhang, Z.-H., Liu, E.-H., & Guo, L. (2019). Evaluation of natural deep eutectic solvents for the extraction of bioactive flavone C-glycosides from Flos Trollii. Microchemical Journal, 145, 180–186.
Doughari, J. H. (2012). Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic agents. In Phytochemicals-A global perspective of their role in nutrition and health. InTechOpen.
Godlewska, K., Pacyga, P., Najda, A., & Michalak, I. (2023). Investigation of chemical constituents and antioxidant activity of biologically active plant-derived natural products. Molecules, 28(14), 5572.
Islam, M., Prottay, A. A. S., Sultana, I., Al Faruq, A., Bappi, M. H., Akbor, M. S., Asha, A. I., Hossen, M. M., Machado, P. E. M., & Junior, I. J. S. (2023). Phytochemical screening and evaluation of antioxidant, anti-inflammatory, antimicrobial, and membrane-stabilizing activities of different fractional extracts of Grewia nervosa (Lour.) Panigrahi. Food
Kren, V., & Martínková, L. (2001). Glycosides in medicine:“The role of glycosidic residue in biological activity.” Current Medicinal Chemistry, 8(11), 1303–1328.
KUBER, B. R. (2023). Phytochemical screening and Fourier Transform IR Analysis of Ficus sagittifolia (Warburg Ex Mildbread and Burret) stem bark. International Journal of Pharmacy Research & Technology (IJPRT), 13(1), 73–78.
Kukreti, N., Prakash, H., Verma, A., & Kumar, P. (n.d.). PRELIMINARY PHYTOCHEMICAL SCREENING OF AQUEOUS EXTRACT OF FICUS BENGALENSIS LEAF BUD.
Mendoza, N., & Silva, E. M. E. (2018). Introduction to phytochemicals: secondary metabolites from plants with active principles for pharmacological importance. Phytochemicals: Source of Antioxidants and Role in Disease Prevention, 25.
M Varoni, E., Lodi, G., Sardella, A., Carrassi, A., & Iriti, M. (2012). Plant polyphenols and oral health: old phytochemicals for new fields. Current Medicinal Chemistry, 19(11), 1706–1720.
Omojate Godstime, C., Enwa Felix, O., Jewo Augustina, O., & Eze Christopher, O. (2014). Mechanisms of antimicrobial actions of phytochemicals against enteric pathogens–a review. J Pharm Chem Biol Sci, 2(2), 77–85.
Patel, V., & Patel, R. (2016). The active constituents of herbs and their plant chemistry, extraction and identification methods. J Chem Pharm Res, 8(4), 1423–1443.
Parmar, M. N. (n.d.). Sustainable Development Goal-3 “Ensure Healthy Lives and Promote well-being For at all Ages.”
Rodriguez-Garcia, A., Hosseini, S., Martinez-Chapa, S. O., & Cordell, G. A. (2017). Multi-target activities of selected alkaloids and terpenoids. Mini-Reviews in Organic Chemistry, 14(4), 272–279.
Maheshwaran, L., Nadarajah, L., Senadeera, S., Ranaweera, C. B., Chandana, A. K., & Pathirana, R. N. (2024). Phytochemical Testing Methodologies and Principles for Preliminary Screening/Qualitative Testing. Asian Plant Research Journal, 12(5), 11–38.
Mocan, A., Zengin, G., Simirgiotis, M., Schafberg, M., Mollica, A., Vodnar, D. C., Crişan, G., & Rohn, S. (2017). Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 153–168.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237.
Bioscience, 54, 102933.
Sarkar, B. R. (n.d.). Phytochemical Screening and Proximate exploration of Diapensia himalacia leaf extract.
Saini, R. K., Sivanesan, I., & Keum, Y. S. (2016). Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech, 6(2), 203. https://doi.org/10.1007/s13205-016-0526-3
Selwal, N., Rahayu, F., Herwati, A., Latifah, E., Suhara, C., Suastika, I. B. K., Mahayu, W. M., & Wani, A. K. (2023). Enhancing secondary metabolite production in plants: Exploring traditional and modern strategies. Journal of Agriculture and Food Research, 14, 100702.
Ugwuona, F. U. (2014). Phytochemical composition, antioxidant and antimicrobial properties of four Nigerian spices. Ph. D. Thesis, Department of Food Science and Technology, Faculty of ….
Yashin, A., Yashin, Y., Xia, X., & Nemzer, B. (2017). Antioxidant activity of spices and their impact on human health: A review. Antioxidants, 6(3), 70.
Copyright (c) 2024 Too Lily Chepkemoi, Jared Owiti Yugi, PhD, Joyce Jepkorir Kiplimo, PhD
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