Molecular Detection of Aspergillus Species from Food Sold in Amassoma, Nigeria
Fungal infections are quite common in humans and range from common, superficial infections to invasive and life-threatening infections. With at least 12 phyla and several fungal species globally, more than 500 species are expected to be infectious to man. One such is the ubiquitous Aspergillus species that mainly causes infection in immunocompromised individuals and those with underlying pulmonary disease. This study compared traditional identification methods and molecular biology techniques to isolate Aspergillus species from food samples sold in the University community. Some selected food samples frequently purchased in the community were analysed for the presence of Aspergillus species using both traditional and molecular biology techniques. Amplification of the ITS region of the rRNA genes of the isolate was performed using the ITS gene. A specie identified as Aspergillus niger was phenotypically identified as Aspergillus sydowii, and another species identified as Aspergillus flavus phenotypically was identified as Aspergillus fumigatus genotypically. The results demonstrate that traditional methods might be misleading, especially to a non-taxonomist. Genotypic identification proved to be more reliable and less time-consuming than phenotypic identification
Balajee, S. A., Kano, R., Baddley, J. W., Moser, S. A., Marr, K. A., Alexander, B. D., ... & Chiller, T. (2009). Molecular identification of Aspergillus species collected for the transplant-associated infection surveillance network. Journal of clinical microbiology, 47(10), 3138-3141. https://doi.org/10.1128/JCM.01070-09
Bellemain, E., Carlsen, T., Brochmann, C., Coissac, E., Taberlet, P., & Kauserud, H. (2010). ITS as an environmental DNA barcode for fungi: an in-silico approach reveals potential PCR biases. BMC microbiology, 10, 1-9. https://doi.org/10.1186/1471-2180-10-189
Bouzani, M., Ok, M., McCormick, A., Ebel, F., Kurzai, O., Morton, C. O., ... & Loeffler, J. (2011). Human NK cells display important antifungal activity against Aspergillus fumigatus, which is directly mediated by IFN-γ release. The Journal of Immunology, 187(3), 1369-1376. https://doi.org/10.4049/jimmunol.1003593
Cordasco, E. M., Demeter, S. L. & Zenz, C. (Eds.). (1995). Environmental respiratory diseases. Van Nostrand Reinhold.
Fida, M., Khalil, S., Abu Saleh, O., Challener, D. W., Sohail, M. R., Yang, J. N., Pritt, B. S., Schuetz, A. N., & Patel, R. (2021). Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice. Clinical Infectious Diseases, 73(6), 961-968. https://doi.org/10.1093/cid/ciab167
Fosses Vuong, M., Hollingshead, C. M., & Waymack, J. R. (2023). Aspergillosis. National Library of Medicine. National centre for Biotecnology information.
Hocking, A. D. (2006). Aspergillus and related teleomorphs. In: Blackburn, C. D. W. (Ed), Food Spoilage Microorganism (451-487). England: Woodhead Publishing.
Janda, J. M., & Abbott, S. L. (2007). 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls. Journal of Clinical Microbiology, 45(9), 2761- 2764. https://doi.org/10.1128/JCM.01228-07
Manaka, A., Tokue, Y. & Murakami, M. Comparison of 16S ribosomal RNA gene sequence analysis and conventional culture in the environmental survey of a hospital. J Pharm Health Care Sci 3, 8 (2017). https://doi.org/10.1186/s40780-017-0074-y
Moreira, Filipe Mercês and Pereira, Pamalla de Araujo and de Miranda, Rebeca Vitória da Silva Lage and dos Reis, Cristhiane Moura Falavina and Braga, Lygia Maria Paulo da Silva and de Andrade, Joyce Modesto and do Nascimento, Luciane Gomes and Mattoso, Josiane Machado Vieira and Forsythe, Stephen James and da Costa, Luciana Veloso and Brandão, Marcelo Luiz Lima (2023). Evaluation of MALDI-TOF MS, Sequencing of D2 LSU rRNA and Internal Transcribed Spacer Regions (ITS) for the Identification of Filamentous Fungi Isolated from a Pharmaceutical Facility. http://dx.doi.org/10.2139/ssrn.4407581
Mousavi, B., Hedayati, M., Hedayati, N., Ilkit, M., & Syedmousavi, S. (2016). Aspergillus species in indoor environments and their possible occupational and public health hazards. Current Medical Mycology, 2(1), 36-42. https://doi.org/10.18869/acadpub.cmm.2.1.36
Pathakumari, B., Liang, G., & Liu, W. (2020). Immune defence to invasive fungal infections: A comprehensive review. Biomedicine & Pharmacotherapy, 130, 110550. https://doi.org/10.1016/j.biopha.2020.110550
Pitt, J.I., 1994. The current role of Aspergillus and Penicillium in human and animal health. Journal of medical and veterinary mycology, 32(sup1), pp.17-32. https://doi.org/10.1080/02681219480000701
Rodríguez, A., Rodríguez, M., Andrade, M. J., & Córdoba, J. J. (2015). Detection of filamentous fungi in foods. Current Opinion in Food Science, 5, 36-42. https://doi.org/10.1016/j.cofs.2015.07.007
Vuong, N.N., Hammond, D. and Kontoyiannis, D.P., 2023. Clinical Uses of Inhaled Antifungals for Invasive Pulmonary Fungal Disease: Promises and Challenges. Journal of Fungi, 9(4), p.464.
Walsh, T. J., & Dixon, D. M. (1996). Spectrum of mycoses. Medical microbiology, 919-925.
Copyright (c) 2023 Ebidor Lawani-Luwaji, Alade Tolulope, Kalama Confidence
This work is licensed under a Creative Commons Attribution 4.0 International License.