Developing Sustainable Wood Preservatives: The Antifungal Efficiency of Afzelia quanzensis Welv. and Androstachys johnsonii Prain Sawdust Extracts from Mozambique

Alberto António Manhiça; Ernesto Uetimane Júnior; Mohamed Jebrane; Peter R. Gillah

doi:10.37284/eajfa.7.1.2381

Alberto António Manhiça Sokoine University of Agriculture
Ernesto Uetimane Júnior, PhD Eduardo Mondlane University
Mohamed Jebrane, PhD Swedish University of Agricultural Sciences
Peter R. Gillah, PhD Sokoine University of Agriculture

DOI: https://doi.org/10.37284/eajfa.7.1.2381

Keywords: Sawdust, Wood Extractives, Wood Durability, Wood Destroying Fungi, Antifungal Properties

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Abstract

This study aimed to both develop and assess the antifungal efficacy of extractives derived from different sets of sawdust originating from two hardwood species, namely chanfuta (Afzelia quanzensis Welv.) and mecrusse (Androstachys johnsonnii Prain). These hardwood species possess inherent characteristics of high natural durability. The primary objective was to use these extractives to impregnate perishable wood species, enhancing their durability and augmenting their commercial value. Collected wood sawdust was initially sieved to remove residues and unwanted materials and conditioned until 12% moisture content. Subsequently, the sawdust was Soxhlet-extracted using a mixture of organic solvents, including acetone, ethanol, and toluene. After evaporating the solvents, the resulting extractives were used to prepare a preservative solution with ethanol and acetone. The extractive solutions from each wood species were mixed with malt extract agar and then diluted to various concentrations: 0.125 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1.25 mg/mL, 2.0 mg/mL, and 2.5 mg/mL. For each concentration, fungal plugs from the periphery of actively growing cultures were moved to the centre of Petri dishes and placed in controlled laboratory conditions for incubation. The antifungal efficacy was assessed against specific wood-decaying fungi, including brown rot fungi (Coniophora puteana, Postia placenta, Lentinus lapideus) and white rot (Trametes versicolor). The evaluation involved daily monitoring of the linear expansion along two perpendicular radii for each fungus expressed as a percentage of the empty Petri dish area. The results indicated that the chanfuta extractives inhibited the growth of brown rot fungi at a concentration of 0.25 mg/mL and white rot fungi at 2.0 mg/mL. Similarly, the mecrusse extractives inhibit brown rot at 0.5 mg/mL and white rot at 2.0 mg/mL. The fatty acids Oleic (C18:1) present in the chanfuta extractives solution and linoleic (C18:2) in mecrusse extractives contributed to restraining the fungal growth. These results suggest that sawdust extractives from both wood species exhibit promising potential for creating environmentally friendly wood preservatives, which, with effective treatments, could extend the lifespan of perishable wood species

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References

Bhat, I. U. H., Khalil, H. P. S. A., Shuib, N. S. and Noor, A. M. (2010). Antifungal activity of heartwood extracts and their constituents from cultivated Tectona grandis against Phanerochaete chrysosporium. Wood Research. 55(4); 59-66.

Brocco V. F., Paes, J. B. and Da Costa, L. G. (2015). Potential of teak heartwood extractives as a natural preservative against Nasutitermes corniger termite. The Inter. Res. Group on Wood Preserv., Doc. No. IRG/WP 15-30666.

Broda, M. (2020). Natural compounds for wood protection against Fungi – a Review. Molecules 2020, 25, 3538.

Chang, S. T., Wang, S. Y., Wu, C. L., Su, Y. C., Kuo, Y. H (1999). Antifungal compounds in the ethyl acetate soluble fraction of the extractives of Taiwania (Taiwania cryptomerioides Hayata) heartwood. Holzforschung 53:487-490.

Chittenden, C. and Singh, T. (2011). Antifungal activity of essential oils against wood degrading fungi and their applications as wood preservatives. International Wood Products Journal (2011) vol. 2, No 1, 44-48.

Clausen, C. A., Coleman, R. D. and Yang, V. W. (2010). Fatty Acid-based formulation for wood protection against mold sapstain. Forest Products Journal. 60(3):301-304. Doi: 10.13073/0015-7473-60.3.301.

Daniel, G. (2016). Fungal Degradation of Wood Cell Walls. Secondary Xylem Biology. pp. 131-167.

Guimarães, A. and Venâncio, A. (2022). The Potential of Fatty Acids and Their Derivatives as Antifungal Agents: A Review. Toxins 14, 188. https://doi.org/10.3390/toxins14030188.

Jansson, M. B. and Nilvebrant, N. (2009). Wood extractives. In: EK, M., Gellerstedt, G. and Henriksson, G. (Ed.). Wood Chemistry and Biotechnology. Berlin. Germany. pp. 121-145.

Jusoh, I., Henry, A. T., Assim, Z., Ahmad, F. B. and Ujang, S. (2012). Antifungal activities of acetone–soluble Eusideroxylon zwageri and Potoxylon melagangai crude extracts against white rot.The Inter. Res. Group on Wood Preserv., Doc. No. IRG/WP 12-30591.

Khademibami, L. and Bobadilha, G. S. (2022). Recent development studies on wood protection research in Academia: A Review. Front. For. Change 5:793177. Doi: 10.3389/ffgv.2022.793177.

Kilic, A. and Niemz, P. (2010). Extractives in some tropical woods. Eur. J. Wood Prod. https://doi.org/10.1007/s00107-010-0489-8.

Kržišnik, D and Gonçalves, J. (2023). Environmentally Conscious Technologies using Fungi in a climate-changing world. Earth 4(1): 66-77.

Liibert, L., Treu, A. and Meier, P. (2011). The Fixation of new alternative wood protection systems using oil treatment. Materials Science 17(4): 1392-13920.

Manan, N. A., Jusoh, I. and Pa’ee, F. (2022). Antifungal activities of Neobanocarpus heimii (Cengal) heartwood extracts on Trametes versicolor and Coniophora puteana. Journal of Tropical Biodiversity and Biotechnology. Vol. 7, Issue (3) jtbb69942. Doi: 10.22146/jtbb.69942.

Mburu, F., Dumaçay, S., Thévenon, M. F. and Gérardin, P. (2007). On the Reasons of Prunus africana natural durability. The Inter. Res. Group on Wood Preserv., Doc. No. IRG/WP 07-10611.

Meena, R. K. (2022). Hazardous effect of chemical wood preservatives on the environmental condition, ecological biodiversity, and human being and its alternatives through different botanicals: A Review. Environment and Ecology 40 (3): 1137-1143.

Miranji, E. K., Kipkemboi, P. K. And Kibet, J. K. (2022). A Review of toxic metals and hazardous organics in wood treatment sites and their etiological implications. Journal of Chemical Reviews. 4 (1), 40-66.

Mohareb, A., Sirmah, P., Desharnais, S., Durmarçay, S., Pétrissans, M. and Gerardin, P. (2010). Influence of extractives on the durability of Cupressus lusitanica heartwood. The Inter. Res. Group on Wood Preserv., Doc. No. IRG/WP 10-10716.

Movahed, A. S. (2012). Chemical Interactions between Fatty Acids and Wood Components during the oxidation process. Doctoral Thesis. KTH Chemical Science and Engineering. ISBN 978-91-7501-470-8. Stockholm. 56p.

Özgenç, Ö., Durmaz, S., Yildiz, Ü. C. and Erişir, E. (2017). A comparison between some wood bark extracts: Antifungal activity. Kastamonu Univ., Journal of Forestry Faculty. 17(3):502-508.

Randall, C. (2000). Management of wood-destroying pests: A guide for commercial applicators, category 7B. Extension Bulletin E-2047. Michigan State University Extension

Sablík, P., Giagli, K., Paril, P., Baar, J. and Rademacher, P. (2016). Impact of extractive chemical compounds from durable wood species on fungal decay after impregnation on non-durable wood species. European Journal of Wood Production 74: 231-236.

Shao, D., He, J., Lu, J. Wang, Q. Chang, L. Shi, S. R. and Bing, T. H. (2019). Effects of sawdust thickness on yellow broilers' growth performance, environmental condition, and welfare quality. Poultry Science, 94(1), 1-6.

Sluiter, A., Ruiz, R., Scarlata, C., Sluiter, J. And Templeton, D. (2008). Determination of Extractives in Biomassa. Laboratory Analytical Procedures (LAP). NREL (National Renewable Energy Laboratory) Technical Report. 12p.

Udokpoh, U. and Nnaji, C. (2023). Reuse of sawdust in Development countries in the light of sustainable Development Goals. Recent Progress in Materials,5(1): 0006; doi: 10.21926/rpm.2301006.

Uma, G. S., Sharme, L. and V. R. (2023). The Role of Insects in Nutrients Cycling: Unsung Heroes of Ecosystem Health. Vigyan Varta 4(9):40-42.

Vek, V. Kerzic, E., Poljansek, I, Eklund, P., Humar, M and Oven, P. (2021). Wood Extractives of Silver Fir and Their Antioxidant and Antifungal Properties. Molecules 2021,26,6412. http://doi.org/10.3390/molecules.26216412

Verkasalo, E., Möttönen, V., Roitto, M., Vepsäläinen, J., Kumar, A., Ilvesniemi, H., Siwale, W., Julkunen-Tiitto, R., Raatikainen, O. and Sikanen, L. (2021). Extractives of stemwood and sawmill residues of Scots Pine (Pinus sylvestris L.) for biorefining in four climate regions in Finland – Phenolic and resin acid compounds. Forests, 12, 192. https://doi.org/10.3390/f12020192.

Voda, K., Boh, B., Vrtacnik, M. and Pohleven, F. (2003). Effect of the antifungal activity of oxygenated aromatic essential oil compounds on the white-rot Trametes versicolor and brown rot Coniophora puteana. International Biodeterioration and Biodegradation 51 (2003) 51-59.

Woźniak, M. (2022). Antifungal Agents in Wood Protection -A Review. Molecules 27: 6392. https://doi.org/10.3390/molecules 27196393.

Xia, Y. and Jia, L. (2023). Wood degradation by Fungi and environmentally benign wood preservatives. http://dx.doi.org/10.5772/intechopen.112033.

Yin, X., Huang, A., Zhang, S., Liu, R. and Ma, F. (2018). Identification of three Dalbergia species based on differences in extractive components. Molecules 23: 21-63.

Zabel, R. A. and Morrell, J. J. (2020). In: Wood deterioration agents. Wood Microbiology. Doi: 10.1016/b978-0-12-819465-200002-4.